Bone Grafts

Bone Grafts

Bone grafts are biological scaffolds and support structures. They can be autografts (patient's own bone), allografts (from a donor) or alloplastic. This article details their indications, properties and types of grafts.


Bone Grafts

💛
5 Key Points

1. Bone grafts are autologous (humans) or alloplastic (engineered)
2. Autologous grafts are autografts (patient) or allografts (cadaver)
3. They are incorporated through osteoinduction, -conduction, -genesis
4. Cortical grafts support, and cancellous allow bone regrowth.
5. Graft survival depends on type of graft, fixation and patient factors.


Indications for bone grafts

Bone grafts provide support, encourage bone regeneration and assist fracture healing. 

There is a diverse array of bone grafting indications. Generally speaking, bone grafts and their substitutes are used for:  

  • Structural support by filling bone gaps secondary to trauma or resection
  • Improve healing of fractures with delayed or non-unions.
  • Assist in arthrodeses and spinal fusions


Bone Graft Properties

Bone grafts have one or more of the following properties: osteoconductive, osteoinductive, and osteogenic.

The biological property of bone focuses on 3 areas - osteoconduction, osteoinduction and osteogenesis. This can be visualised in the image below

Bone grafts have different amounts of osteoconduction, osteinduction and osteogenesis. There are the biologiical properties of bone and bone grafts
Bone Graft Characteristics

Osteoconductive

  • Osteoconductive material is a structural framework for bone growth.
  • For example, demineralized bone matrices (DBMs)
  • More apparent in cancellous bone grafts.

Osteoinductive

  • Osteoinductive material stimulates osteogenesis and induces stem cells
  • For example, bone morphogenetic protein (BMP) arises from the TGF-B family.

Osteogenic

  • Osteogenic material provides bone-producing cells (mesenchymal stem cells, osteoblasts, and osteocytes) to produce more bone.
  • For example, an autologous bone graft is the only bone graft that contains live mesenchymal precursor cells

💡
Fun Fact: cancellous bone has a greater ability than cortical bone to form new bone due to its larger surface area


Classification of Bone Grafts

There are several different ways to classify the different types of bone grafts. This image below provides a comprehensive review.

Bone grafts can be autologous, alloplastic, cortical and cancellous
Classification of Bone Grafts

💡
Tip: Autografts can be further subclassified into vascularised and non-vascularised. 


Autologous Bone Autografts

Autologous bone autografts are cortical, cancellous or corticocancellous bone from the patient that can be vascularised and non-vascularised. They retain all biological properties of bone. 

Indications

Bone Autografts are indicated in bone defects <6cm and:

  • Well-vascularized bed
  • Adequate soft tissue cover
  • Absence of infection

Biological Properties

Bone Autografts are considered the "gold standard" because of their biological properties:

  • Osteogenic and osteoinductive and osteoconductive properties.
  • Less immunogenic than other options.

Types of Autografts

Autografts can be cortical, cancellous or a combination. Each option has its own limitations and advantages. Generally speaking, cortical is used when large defects require structural support.  

Cortical Bone Grafts

  • Less biologically active than cancellous
  • More structural support than cancellous.
  • Slower incorporation due to remodelling of Haversian canals (up to 2 months)
  • Heals primarily by osteoconduction.  

Cancellous Bone Grafts

  • An osteoconductive 3D scaffold with minimal structural support
  • A high number of osteoblasts and growth factors.
  • Rapid incorporation via creeping substitution (within 2 weeks)
  • Heals primarily by osteogenesis.

Donor Sites

Donor site options for autografts include:

  • Iliac Crest - commonly used  and can give cancellous and cortical bone
  • Fibula - for a vascular autograft (example - fibula graft with peroneal artery)
  • Ribs - for vascular autografts.  
  • Distal radius
  • Bone marrow aspirate

💡
Tip: Autografts are considered the "gold standard". They can be cortical, cancellous or a combination. 

Bone Allografts

Allografts are bone grafts from a (cadaver) donor that can be fresh, freshly frozen or freeze-dried bone derived from a donor.

Bone allografts are osteoconductive only due to a lack of viable cells. It is limited by its immunogenicity.

💡
Tip: Autografts are considered the "gold standard" of bone grafting. 


Alloplastic Bone Substitutes

There is an array of "synthetic" and "engineered biological" bone substitutes that provide structural support.

Alloplastic bone substitutes have a primary role in support. They are primarily osteoconductive with little osteogenic or osteoconductive properties

Here are a few examples:

  • Demineralised bone matrix has an osteoinductive and osteoinductive capacity without structural support. It contains collagen, bone morphogenetic proteins, transforming growth factor-beta, residual calcium but does not contain mesenchymal precursor cells.
  • Calcium Hydroxyapatite: brittle but limited resorption.
  • Methylmethacrylate (MMA): non-resorbable, high density, "bone cement", structural support with osteoconductive properties only.
  • Bone morphogenetic proteins (BMP): osteoinductive only

💡
Tip: Alloplasts vary in their osteoconduction, inductive properties, and resorption rates. 


Bone Graft Healing and Incorporation

Autologous bone grafting incorporates through 3 main mechanisms: osteoconduction, osteoinduction, and osteogenesis.

After haematoma formation and an initial inflammatory period, in which necrotic debris stimulates chemotaxis, bone grafts become incorporated into their new bony surroundings. In a similar way, skin grafting becomes adherent to a wound bed.  This is visualised in the image below.

It is different from the standard bone healing process.

a bone graft healings and incorporates into the recipient bed through osteoconduction, osteoinduction and osteogenesis
Bone Graft Incorporation - Osteoconduction, Induction and Genesis

Osteoconduction  

  • The bone graft acts as a scaffold for bone growth via creeping substitution
  • It provides a matrix for bone growth that enables a blood supply and osteoprogenitor cells to survive.
  • Occurs in cortical bone, calcium hydroxyapatite, and less so in cancellous bone.

Osteoinduction

  • Bone graft growth factors stimulate osteogenesis in the host tissue.
  • Osteocytes are formed from the differentiation of the recipient site's mesenchymal cells
  • Strongly influenced by BMPs.
  • This process is seen in cancellous bone grafts and demineralised bone matrix

Osteogenesis

  • Osteoblasts that survive in the bone graft directly produce new bone.
  • A faster process in vascularized bone grafts as they do not undergo creeping substitution.
  • Occurs primarily in vascularized bone grafts and cancellous bone.

💡
Definition: Creeping substitution is the osteoclastic resorption of dead bone from the allograft and its replacement by new living bone made by osteoblasts from the host.


Bone Graft Survival

Bone grafts are similar to skin grafts in that they are influenced by patient factors, graft factors and the recipient site.

Patient Factors

A medically optimised patient will always have a better outcome for their bone grafts. For example, diabetes control, smoking cessation and adjusting immunosuppression drugs.

Graft Factors

When it comes to the graft, there are so many variables that can have a positive and negative influence on its healing. Here are some key points:

  1. Type of Bone Graft
  • Periosteum on bone grafts reduces the amount of resorption.
  • Cancellous bone grafts are more readily revascularized than cortical.

2. Inset of Bone Graft

  • Orthotopic: if the graft is placed in a normal bone location then there will be less bone resorption.
  • Fixation: bone graft fixation improves survival.
  • Quality of recipient: infection and radiotherapy reduce graft survival.

💡
Fun Fact: A bone graft is considered to be incorporated when there is no abrupt histological change between the host bone and the graft


Flashcards

Evidence-based flashcards are designed for active recall and spaced repetition of the verified information you are currently studying.

They are continually updated and are for thePlasticsPro users. 

This section is for supporters of thePlasticsFella's mission

Join the club
Already have an account? Sign in
Great! Next, complete checkout for full access to thePlasticsFella.
Welcome back! You've successfully signed in.
You've successfully subscribed to thePlasticsFella.
Success! Your account is fully activated, you now have access to all content.
Success! Your billing info has been updated.
Your billing was not updated.